6-Metatranscriptomics

Request edit access

JavaScript isn't enabled in your browser, so this file can't be opened. Enable and reload.

One of the main issues in metatranscriptomics is

to obtain high-quality RNA for library preparation

recover full-length transcripts

ribosomal RNA removal

Don't know.

What is one of the difficulties associated with analysis of replicates in gut samples?

Microbial communities have highly variable in composition among samples

Data normalization cannot be performed in metatranscriptomics

It is not relevant to perform biological or technical replicates in metatranscriptomics

Don't know.

Clear selection

What is one of the limitations for rRNA subtraction during sample preparation?

The high quantity of rRNA makes difficult to completely remove it

Presence of secondary structures in rRNA makes difficult its removal from samples

The rRNA sequence of some species is unknown and it is difficult to design probes for an efficient subtraction

Don't know.

Clear selection

How can you compensate for variation in species abundance in the comparison between samples?

Changes in abundance of each species can be compensated with genome-centric metagenomics

It is not needed to correct for changes in species abundance

Changes of species abundance is corrected during calculation of RPKM

Don't know.

Clear selection

Is it possible in metatranscriptomics to analyze shotgun reads without performing assembly?

Yes using pipelines like HUMANN2

No, you must assemble the reads before analysing them

Yes using software like CENTRIFUGE or KRAKEN

Don't know

Clear selection

Statistical analysis and identification of differentially expressed genes can be performed using

RPKM calculation and analysis of log2 values between different samples

a t-test for each gene taking into account results for replicates

software like DESeq2 that model reads counts and dispersion with negative binomial distribution

Don't know.

Clear selection

Submit

Clear form

This content is neither created nor endorsed by Google. - Terms of Service - Privacy Policy

Does this form look suspicious? Report