Quiz 4 Enzymes associated with DNA

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Includes enzymes associated with DNA, DNA replication in bacteria

Name *

1 point

ID Number *

1 point

Email ID *

1 point

The enzyme responsible for DNA replication during bacterial reproduction *

1 point

DNA Polymerase I

DNA Polymerase II

DNA Polymerase III

DNA Polymerase IV

Which statement is incorrect? *

1 point

PolIII has 3`-5` proof reading capability

PolIII has 5`-3` exonuclease activity

PolIII polymerizes at 3`-OH terminus

PolIII requires primers for polymerization

DNA Polymerase adds new nucleotide at this position *

1 point

3'OH

5'PO4

5'OH

3'PO4

5' to 3' exonculease activity of DNA polymerase is known as _________ *

1 point

Nick translation

Proof reading

Transcription

Priming

The direction of DNA Polymerization is ______ *

1 point

5` to 3'

3' to 5'

The enzyme responsible for DNA repair *

1 point

DNA Polymerase I

DNA Polymerase II

DNA Polymerase III

DNA Polymerase V

This subunit of DNA Polymerase III is responsible for proof reading *

1 point

Alpha

Beta

Epsilon

Gamma

Observe the DNA Polymerase complex (PolIII) and indicate which statement is incorrect? *

1 point

Alpha - Polymerase; Epsilon - Proof reading; Theta and Toe - Dimerization; Delta - Clamp loading

Alpha - Polymerase; Epsilon - DNA Clamp; Theta and Toe - Dimerization; Delta - Proof reading

Alpha - Polymerase; Epsilon - Nick translation; Theta and Toe - Dimerization; Delta - Proof reading

Alpha - Polymerase; Epsilon -Proof reading; Theta and Toe - Dimerization; Delta - Nick translation

DnaB refers *

1 point

Helicase

Primase

Ligase

Topoisomease

Melting of DNA refers *

1 point

Depolymerization of nucleotides

Removal of Hydrogen bonds between strands

Removal of N-bases from the nucleotide

Removal of OH from the sugar

The enzyme responsible for strand separation during DNA replication *

1 point

Primase

Topoisomerase

Helicase

Ligase

To start the DNA polymerization, Pol enzymes need *

1 point

ATP

Primers

Dimerization

Klenow fragment

The DNA-protein complex formed during initiation period of DNA replication *

1 point

Primosome

Replisome

Ribosome

Nucleosome

DnaG codes for *

1 point

DNA Primase

DNA Helicase

DNA Ligase

DNA Polymerase

Enzyme responsible for supercoiling of DNA *

1 point

DNA ligase

DNAse

DNA topoisomerase

DNA helicase

DNA ligase is an ATP-driven enzyme responsible for joining of two strands of DNA *

1 point

True

False

DNAse refers Polymerases *

1 point

False

True

The enzyme responsible for cutting the DNA at specific site are known as ________ *

1 point

Endonuclease

DNase

Exonuclease

DNA lyase

Topoisomerase II is also known as *

1 point

Gyrase

TOPO

Helicase

Lygase

Bacterial DNA replication is *

1 point

semi-conservative method

dis-continuous in one strand

having lagging and leading strands

All these are applicable

The precursor required for DNA replication is *

1 point

Nucleosides

Nucleotide triphosphate

Mono nucleotide phosphate

Nucleotide monophosphate

Discontinuously synthesized DNA fragments are present in leading strand *

1 point

True

False

A oligomeric DNA fragment required for starting of DNA Polymerization is known as *

1 point

Primer

Deoxynucleotide

Inducer

Prion

Which sentence is incorrect? *

1 point

DNA replication in bacteria is bidirectional

DNA replication is associated with several enzymes

The speed of polymerization is 800 bp per minute

Bacterial DNA replication is known as theta replication

Okasaki fragments are *

1 point

DNA fragments synthesized in lagging strand

DNA fragments synthesized in leading strand

RNA fragments synthesized for DNA replication

RNA fragments synthesized for DNA repair

Mostly, bacterial genome processes __________ numbers of origin of replication (ori) *

1 point

One

two

four

More than twp

Identify the components of replication fork *

1 point

A - Leading strand; B - Lagging strand; C - Discontinuous; D - Continuous; E - Okasaki

A - Leading strand; B - Lagging strand; C - Continuous; D - Discontinuous; E - Okasaki

A - Leading strand; B - Lagging strand; C - Okasaki; D - Discontinuous; E - Primers

A - Leading strand; B - Lagging strand; C - Klenow fragments; D - Discontinuous; E - Okasaki

Identify the components *

1 point

A - Primosome; B - Helicase; C - Okasaki fragment

A - Replisome; B - Helicase; C - Klenow fragment

A - Replisome; B - Helicase: C - Okasaki fragment

A - Primosome; B - Helicase: C - Klenow fragment

Observe the bacterial DNA replication process carefully and answer the questions (A1 to A5)

A1 - The point present as "A" is site of origin which denoted as ori *

1 point

True

False

A2 - "B" denotes that DNA replication is bidirectional and semi-conservative *

1 point

True

False

A3 - For "C", which statement is incorrect? *

1 point

The speed of replication for both replication fork will be equal.

One fork's leading strand is lagging for another.

This semi-conservative replication is referred as "theta" replication.

One copy of DNA is from parental and another one is newly synthesized

A4: Diagram "D" is called as *

1 point

DNA dimers

Concomitant DNA

Catenane DNA

Chromatid DNA

A5. DNA replication time and binary fission time are equal for bacteria (Ref. "E") *

1 point

True

False

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