MicrobiomeDB - Learn How

Step 1 - choose a set of samples

A. Go to the "Identify Samples based on Host/Sample Metadata" page

B. Use that search to find samples of interest.

First, choose the datasets of interest

Next, navigate to a filter, and select samples of interest

As needed, navigate to and adjust other filters

Finally, click Get Answer

Step 2. Graph your samples

Step 1 - choose a set of samples

A. Go to the "Identify Samples based on Host/Sample Metadata" page

From the home page, click Get Started, or, from the menu bar, click Host/Sample Metadata

B. Use that search to find samples of interest.  

In that search, you will use one or more filters to find your samples of interest.  Each filter allows you to constrain the selected samples based on a particular metadata characteristic.

First, choose the datasets of interest:

Next, navigate to a filter, and select samples of interest

In the example below, the Environmental (Material) filter is open.  Use that filter to choose samples based on the environmental material.  In this case, dust and saliva are selected.  This has reduced the selected set of samples from 13565 to 2290.

As needed, navigate to and adjust other filters

Use the panel on the left to navigate to additional filters, if needed.  For example, choosing none in the Pets and Farm Animals filter reduces the samples from 2290 to 615.  You can see from the bar to the right of none that about a third of the samples (the red part of the bar) with none were from dust or saliva (your previously applied filters).

Finally, click Get Answer

After you are done using various filters to select your samples, click Get Answer at the bottom of the search page. This will take you to the Results Page.

Step 2. Graph your samples

In the results page, you can see a listing of the samples you selected.

Click the blue Analyze Results button to access the Shiny-based OTU Abundance analysis tool.

Click on the OTU Abundance icon:

And then, the Run Analysis button