Supplementary Data
Table S1 Correlation of T cell subset transcription factors and cytokines in peripheral blood in cases (n=13)
| T-bet | GATA-3 | Foxp3 | RORγt | IFNγ | TGFβ | TNFα | IL-4 | IL-5 | IL-10 |
T-bet | - |
|
|
|
|
|
|
|
|
|
GATA-3 |
| - |
|
|
|
|
|
|
|
|
Foxp3 |
| 0.7418 | - |
|
|
|
|
|
|
|
RORγt |
|
|
| - |
|
|
|
|
|
|
IFNγ | 0.6217 |
|
| 0.5879 | - |
|
|
|
|
|
TGFβ |
|
|
|
|
| - |
|
|
|
|
TNFα |
|
|
|
|
|
| - |
|
|
|
IL-4 |
|
|
| 0.7527 | 0.8791 |
|
| - |
|
|
IL-5 | 0.7290 |
| 0.6374 |
|
|
|
|
| - |
|
IL-10 | 0.7923 |
|
|
|
|
|
|
|
| - |
mRNA expression relative to GAPDH of CD4 T cell transcription factors and cytokines from PBMCs were used to look at correlations during infection in cases (before treatment, n=13). Correlations that were statistically significant (P<0.05) are shown.
Table S2 Correlation of T cell subset transcription factors and cytokines in peripheral blood in controls (n=15)
| T-bet | GATA-3 | Foxp3 | RORγt | IFNγ | TGFβ | TNFα | IL-4 | IL-5 | IL-10 |
T-bet | - |
|
|
|
|
|
|
|
|
|
GATA-3 |
| - |
|
|
|
|
|
|
|
|
Foxp3 |
|
| - |
|
|
|
|
|
|
|
RORγt |
|
|
| - |
|
|
|
|
|
|
IFNγ |
|
|
|
| - |
|
|
|
|
|
TGFβ |
|
|
|
|
| - |
|
|
|
|
TNFα |
| 0.5455 |
|
|
|
| - |
|
|
|
IL-4 |
|
| 0.7677 | 0.5899 |
|
|
| - |
|
|
IL-5 |
|
|
|
|
|
|
|
| - |
|
IL-10 |
|
|
|
|
|
|
|
|
| - |
mRNA expression relative to GAPDH of CD4 T cell transcription factors and cytokines from PBMCs were used to look at correlations in controls (n=15). Correlations that were statistically significant (P<0.05) are shown.
Table S3 Correlation of T cell subset transcription factors and cytokines in peripheral blood in controls and cases in HIV seronegative individuals (n=18)
| T-bet | GATA-3 | Foxp3 | RORγt | IFNγ | TGFβ | TNFα | IL-4 | IL-5 | IL-10 |
T-bet | - |
|
|
|
|
|
|
|
|
|
GATA-3 | 0.6754 | - |
|
|
|
|
|
|
|
|
Foxp3 | 0.7429 | 0.7381 | - |
|
|
|
|
|
|
|
RORγt | 0.8961 | 0.5832 | 0.6292 | - |
|
|
|
|
|
|
IFNγ |
|
|
|
| - |
|
|
|
|
|
TGFβ | 0.6659 | 0.5767 | 0.6159 | 0.7687 |
| - |
|
|
|
|
TNFα |
|
|
|
|
|
| - |
|
|
|
IL-4 |
|
|
| 0.6019 |
|
|
| - |
|
|
IL-5 | 0.9194 | 0.6567 | 0.8133 | 0.7897 |
| 0.6753 |
| 0.5103 | - |
|
IL-10 | 0.9689 | 0.6664 | 0.6973 | 0.8822 |
| 0.6927 |
|
| 0.8688 | - |
mRNA expression relative to GAPDH of CD4 T cell transcription factors and cytokines from PBMCs were used to look at correlations in relation to HIV status (HIV seronegative, n=18) regardless of infection status. Correlations that were statistically significant (P<0.05) are shown.
Table S4 Correlation of T cell subset transcription factors and cytokines in peripheral blood in controls and cases in HIV seropositive individuals (n=9)
| T-bet | GATA-3 | Foxp3 | RORγt | IFNγ | TGFβ | TNFα | IL-4 | IL-5 | IL-10 |
T-bet | - |
|
|
|
|
|
|
|
|
|
GATA-3 |
| - |
|
|
|
|
|
|
|
|
Foxp3 |
| 0.8965 | - |
|
|
|
|
|
|
|
RORγt | 0.7342 |
| 0.7318 | - |
|
|
|
|
|
|
IFNγ |
|
|
|
| - |
|
|
|
|
|
TGFβ | 0.9220 |
| 0.7343 | 0.8584 |
| - |
|
|
|
|
TNFα |
|
|
|
|
|
| - |
|
|
|
IL-4 |
|
|
| 0.6702 |
|
|
| - |
|
|
IL-5 | 0.9220 |
| 0.7343 | 0.8584 |
| 1.0000 |
|
| - |
|
IL-10 |
|
|
|
|
|
|
|
|
| - |
mRNA expression relative to GAPDH of CD4 T cell transcription factors and cytokines from PBMCs were used to look at correlations in relation to HIV status (HIV seropositive, n=9) regardless of infection status. Correlations that were statistically significant (P<0.05) are shown.