Pyrocystis lunula  

(literally, moon-shaped fire cell - cool, huh?)

[Note: If you want to learn more, Patrik will be teaching a Glow-in-the-Dark Dinos! class on Wednesday June 6, 6:30pm at BioCurious. We’ll have more dinos and growth medium to hand out at that time.]

These bioluminescent dinoflagellates (we just call them “dinos”) are single-celled marine algae. They live from sunlight during the day, and give off visible light when disturbed at night.

Your dinos will be happiest in a spot that is well-lit but not too warm (no direct sunlight). They will only glow at night, and they’re brightest 2-4 hours after dark, so you may want to place them in your bedroom, where you can say hello to them before getting into bed.

Let your eyes adapt to the dark for a few minutes, then rock the dinos gently back and forth to make them light up. You’ll be able to see the single cells as they flash in response.

Your pet dinos should be ok in the vial for a couple of weeks. You can grow a fresh batch by pouring them into a bottle of seawater with some additional nutrients. If you want to keep them growing, this should be done every 3-4 weeks. Add one part of the old culture to 3-4 part salt water with nutrients. You can get salt water at your local aquarium store for a few bucks per gallon. Or get some seawater at the beach, boil it for a few minutes and let it cool to room temperature.

For additional nutrients, we’ve tried a number of different plant fertilizers to try mimic the effect of the “professional” mix:

- Miracle Gro Liquid Houseplant Food (15 drops per liter of salt water)

- Miracle Gro Water Soluble All Purpose Plant Food (2.5 mg per liter)

- Alaska Fish Fertilizer 5-1-1 (1/10th of a drop per liter)

The amounts given here are just a suggestion - experiment and let us know what works! You can also buy the additional nutrients online here:

http://florida-aqua-farms.com/secure/agora.cgi?product=MICRO_MACRO_NUTRIENTS

Or buy the growth medium (salt water + nutrients) here:

http://empco.org/edu/index.php/bioluminescent-algae/algal-growth-media/l1-algal-growth-medium.html

Since the dinos live by photosynthesis, they do need access to some CO2. Just like other algae and plants, they use CO2 as their carbon source, and produce O2 in the process. However, they grow slow enough that they can go for a long time with the cap on. If you grow them in a bottle, you may want to blow in the bottle when you say hi to them at night, to give them some extra CO2 from your breath.

For more information:

https://empco.org/empco/images/quickguides.pdf

http://www.lifesci.ucsb.edu/~biolum/organism/dinolab.html

If you would like to learn more about bioluminescence, and maybe tinker with engineering bioluminescent bacteria, come join us for the BioLuminescence Community Project at BioCurious, every Monday evening at 7:30pm. No membership required!

If you have any questions, feedback on how your dinos are doing, or suggestions for how to improve this page, feel free to contact patrikd@gmail.com