Advancing in the application of innovative phytomanagement strategies in polluted sites in southwest Europe
2nd Phy2SUDOE Workshop
Participants had the opportunity to get insights and learn more about phytomanagement of contaminated soils, soil restoration and policy from renowned experts in the fields. The main topics were:
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The implantation of the test plot began in March 2022 with 2 steps. At first, we removed the concrete slab and decompressed the soil to 1 meter deep. During this step, we discovered unknown works (old pipe and metal wells) and PCB high levels that necessitated moving the plot.
In the second time, seedlings and plantings prepared by INREA were transplanted on the plot (see the joint plan). The technical and scientific monitoring will be realized by HPC Environnementec under the supervision of INRAE.
The aim of this meta-study is to assess the long-term effect of Populus sp. phytomanagement on soil biodiversity at different levels of the food web. The idea is to study variables related to ecological restoration dynamics (diversity, interactions, functionality, stability) and try to find common patterns between sites. To this end, metabarcoding measurements will be made of 16S rRNA (bacteria and archaea), ITS (fungi), 18s rRNA and COI (other eukaryotes) genes. Metabarcoding allows the genetic composition of a DNA sample to be determined by PCR amplification of specific fragments, followed by sequencing by high-throughput next-generation sequencing methodologies.
Long-term field trials are implemented at this site (since 2013), a part of the urban Parc aux Angéliques, to assess the phytomanagement of contaminated soils with either grassland or grassland dotted with black poplars. Soil samples (in triplicates) were collected in two field trials (B and F) with grassland and two field trials (D and G) with grassland and poplars to compare the soil microbial communities (work carried out by the Neiker partner) and to investigate the presence of invertebrates (work carried out by the University of Basque Country, partner). Changes in microbial and invertebrate communities are indicators to assess the soil remediation and ecological soil functions underlying ecosystem services.
Soils were collected at 6 stations of the Bordes site (NS4), a phytomanaged former landfill rehabilitated under the supervision of the Pyrénées-Atlantiques County (associated partner CD64, Ch. Paille-Barrère). Soils were assessed in a pot experiment. The aim was to ameliorate the physicochemical soil properties and to reduce the plant exposure to metal(loid)s. Soils were amended with 5% (w/w) compost and compared to untreated soils. Dwarf beans were sowed in potted soils. Germination rate and shoot biomass of dwarf beans were determined. In parallel, the germination of the seed bank was recorded. In general no visible symptom of phytotoxicity was recorded on dwarf beans. The seed bank was relatively high except in station 5. The compost incorporation into the soils was increasing the seed bank and the shoot biomass (due to nutrient supply), and promoted the species richness of the plant community.
Long-term field plots have been implemented at the St Médard d’Eyrans site (S1) to assess the phytomanagement of soils polluted by Cu/PAH with either a short-rotation coppice or a plantation of poplars (Populus nigra L.). In the end of March 2022 (year 14), the INRAE researchers went to this site to collect soil samples in 12 plots with and without poplars. The purpose was to monitor changes in the soil microbial community (work carried out by the Neiker partner) and in the invertebrate community (work carried out by the University of the Basque Country, partner) in line with the presence of poplars in the initial plant community.
In March 2022, the concrete slab was removed on roughly 200 m² at the Durandeau site under the supervision of La Charente County partner and HPC-Envirotec. The 0 – 0.50 m soil layer was loosened. This area displayed high metal(loid), PAH, PCB, and trichloroethylene concentrations in the topsoil. Based on previous pot experiments carried out by INRAE, compost (5% w/w) was incorporated in the topsoil (0 – 25 cm, Jardins de l’Angoumois)
Long-term field plots were implemented at the St Médard d’Eyrans site (S1) to assess the phytomanagement of soils polluted by Cu/PAH with perennial Cu-excluder grasses such as Miscanthus x giganteus. Plots amended with compost (5% w/w) and dolomitic limestone (0.2% w/w) in year 1 were compared to untreated plots.
In mid-June and early July 2021, INRAE researchers were working at the Phy2SUDOE S1 site (St Médard d’Eyrans, France) to measure the maximum shoot length and to harvest winter barley (Hordeum vulgare L. cv. Séduction) and then grain-type intermediate wheatgrass (Thinopyrum intermedium cv Kernza).
Zero-valent iron nanoparticles and organic amendment assisted rhizoremediation of mixed contaminated soil using Brassica napus
Here, we evaluated the effect of the addition of zero-valent iron nanoparticles (nanoremediation) and organic amendments (biostimulation) on the rhizoremediation, using Brassica napus plants, of soil simultaneously contaminated with zinc (2500 mg kg −1) and lindane (100 mg kg−1). We used a factorial design with three factors (amendment, nZVI, plant) to evaluate the impact of the applied remediation actions on lindane and extractable Zn concentrations, as well as on soil health recovery as manifested by the values of different soil microbial indicators.
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Carlos Garbisu Crespo (Coordinator)
Neiker (Instituto Vasco de Investigación y Desarrollo Agrario)
Parque Tecnológico de Bizkaia C/ Berreaga, 1, E-48160 Derio (Bizkaia) Spain
+34 944 034 300
The project Phy2SUDOE (SOE4/P5/E1021) is supported