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Phy2SUDOE Newsletter #3
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Advancing in the application of innovative phytomanagement strategies in polluted sites in southwest Europe


June 2022


Welcome to the third newsletter of the Phy2SUDOE project. This newsletter series provides you with updated information on our project progress, achievements and important forthcoming activities. You may also follow us on our Twitter and Facebook accounts.

Phy2SUDOE (“Advancing in the application of innovative phytomanagement strategies in contaminated areas of the SUDOE space”, SOE4/P5/E1021) is a project funded by the European Regional Development Fund (European Commission) through the Interreg Sudoe Programme. Phy2SUDOE is a continuation of the PhytoSUDOE project, in which a network of sites was formed to be phytomanaged in the SUDOE region.

Phy2SUDOE aims to value sites contaminated with metals-metalloids and/or organic compounds in the SUDOE region through the use of phytomanagement strategies aimed at the generation of ecosystem products and services in these sites, while minimizing the environmental impact of pollutants. could cause. Likewise, this project aims to implement strategies for the conservation of endemic biodiversity typical of some contaminated sites (e.g, plant growth-promoting bacteria, metallurgical flora, etc.) due to their intrinsic and utilitarian value (e.g., biotechnological applications).

Enjoy reading,

Carlos Garbisu
Coordinator of the Phy2SUDOE project.

2nd Phy2SUDOE Workshop

Participants had the opportunity to get insights and learn more about phytomanagement of contaminated soils, soil restoration and policy from renowned experts in the fields. The main topics were:

  • Long-term maintenance and monitoring of phy2SUDOE network sites
  • Phytomanagement options under a variety of soil and climate conditions
  • Conservation of endemic biodiversity in contaminated sites
  • Experience of phytomanagement on the Jules Durandeau site
  • Vitoria-Gasteiz’s Graveras de Lasarte polluted site case study
  • Adaptive design and management practices for habitat restoration plantations and invasive species mitigation

Phy2SUDOE latest news

Jules Durandeau’s phytomanagement facility

The implantation of the test plot began in March 2022 with 2 steps. At first, we removed the concrete slab and decompressed the soil to 1 meter deep. During this step, we discovered unknown works (old pipe and metal wells) and PCB high levels that necessitated moving the plot.

In the second time, seedlings and plantings prepared by INREA were transplanted on the plot (see the joint plan). The technical and scientific monitoring will be realized by HPC Environnementec under the supervision of INRAE.

Meta-study to assess the long-term effect of phytomanagement with Populus sp. on soil biodiversity

The aim of this meta-study is to assess the long-term effect of Populus sp. phytomanagement on soil biodiversity at different levels of the food web. The idea is to study variables related to ecological restoration dynamics (diversity, interactions, functionality, stability) and try to find common patterns between sites. To this end, metabarcoding measurements will be made of 16S rRNA (bacteria and archaea), ITS (fungi), 18s rRNA and COI (other eukaryotes) genes. Metabarcoding allows the genetic composition of a DNA sample to be determined by PCR amplification of specific fragments, followed by sequencing by high-throughput next-generation sequencing methodologies.

INRAE collected soils at the S2 site (Chaban-Delmas) for investigating microbial and invertebrate communities

Long-term field trials are implemented at this site (since 2013), a part of the urban Parc aux Angéliques, to assess the phytomanagement of contaminated soils with either grassland or grassland dotted with black poplars. Soil samples (in triplicates) were collected in two field trials (B and F) with grassland and two field trials (D and G) with grassland and poplars to compare the soil microbial communities (work carried out by the Neiker partner) and to investigate the presence of invertebrates (work carried out by the University of Basque Country, partner). Changes in microbial and invertebrate communities are indicators to assess the soil remediation and ecological soil functions underlying ecosystem services.

Phytotoxicity testing and assessment of seed bank on the Bordes soils

Soils were collected at 6 stations of the Bordes site (NS4), a phytomanaged former landfill rehabilitated under the supervision of the Pyrénées-Atlantiques County (associated partner CD64, Ch. Paille-Barrère). Soils were assessed in a pot experiment. The aim was to ameliorate the physicochemical soil properties and to reduce the plant exposure to metal(loid)s. Soils were amended with 5% (w/w) compost and compared to untreated soils. Dwarf beans were sowed in potted soils. Germination rate and shoot biomass of dwarf beans were determined. In parallel, the germination of the seed bank was recorded. In general no visible symptom of phytotoxicity was recorded on dwarf beans. The seed bank was relatively high except in station 5. The compost incorporation into the soils was increasing the seed bank and the shoot biomass (due to nutrient supply), and promoted the species richness of the plant community.

Sampling and tools for assessing the remediation of soils phytomanaged by poplars at the S1 site (St Médard d’Eyrans)

Long-term field plots have been implemented at the St Médard d’Eyrans site (S1) to assess the phytomanagement of soils polluted by Cu/PAH with either a short-rotation coppice or a plantation of poplars (Populus nigra L.). In the end of March 2022 (year 14), the INRAE researchers went to this site to collect soil samples in 12 plots with and without poplars. The purpose was to monitor changes in the soil microbial community (work carried out by the Neiker partner) and in the invertebrate community (work carried out by the University of the Basque Country, partner) in line with the presence of poplars in the initial plant community.

Set up of a phytomanagement trial at the Durandeau site (NS1)

In March 2022, the concrete slab was removed on roughly 200 m² at the Durandeau site under the supervision of La Charente County partner and HPC-Envirotec. The 0 – 0.50 m soil layer was loosened. This area displayed high metal(loid), PAH, PCB, and trichloroethylene concentrations in the topsoil. Based on previous pot experiments carried out by INRAE, compost (5% w/w) was incorporated in the topsoil (0 – 25 cm, Jardins de l’Angoumois)

Annual harvest of the dried shoots of Miscanthus X giganteus at the St Médard D’Eyrans site (S1)

Long-term field plots were implemented at the St Médard d’Eyrans site (S1) to assess the phytomanagement of soils polluted by Cu/PAH with perennial Cu-excluder grasses such as Miscanthus x giganteus. Plots amended with compost (5% w/w) and dolomitic limestone (0.2% w/w) in year 1 were compared to untreated plots.

INRAE researchers measuring plant parameters at the Phy2SUDOE S1 site (St Médard D’Eyrans)

In mid-June and early July 2021, INRAE researchers were working at the Phy2SUDOE S1 site (St Médard d’Eyrans, France) to measure the maximum shoot length and to harvest winter barley (Hordeum vulgare L. cv. Séduction) and then grain-type intermediate wheatgrass (Thinopyrum intermedium cv Kernza).

New publications

Zero-valent iron nanoparticles and organic amendment assisted rhizoremediation of mixed contaminated soil using Brassica napus

Here, we evaluated the effect of the addition of zero-valent iron nanoparticles (nanoremediation) and organic amendments (biostimulation) on the rhizoremediation, using Brassica napus plants, of soil simultaneously contaminated with zinc (2500 mg kg −1) and lindane (100 mg kg−1). We used a factorial design with three factors (amendment, nZVI, plant) to evaluate the impact of the applied remediation actions on lindane and extractable Zn concentrations, as well as on soil health recovery as manifested by the values of different soil microbial indicators.

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Contact details

Carlos Garbisu Crespo (Coordinator)

Neiker (Instituto Vasco de Investigación y Desarrollo Agrario)

Parque Tecnológico de Bizkaia C/ Berreaga, 1, E-48160 Derio (Bizkaia) Spain

+34 944 034 300

The project Phy2SUDOE (SOE4/P5/E1021) is supported
by the European Regional Development Fund (European Commission)
through the V Interreg Sudoe Programme.

bandera UE