Index of SOPs and Protocols

Standard Operating Procedures for Lab Equipment and Regular Tasks

• Autoclave

• Operation

• SpeedClave Operation
• Yamato SQ500C Operation

• Maintenance

• SpeedClave Manual
• SpeedClave Cleaning and Maintenance (see manual pg 15-16)]  [TBW, see manual]
• Yamato SQ500C Operating Manual
• Yamato SQ500C Service Manual
• Yamato SQ500C additional info and records
• Yamato SQ500C Cleaning and Maintenance

• Spectrophotometer

• Optical Density (OD) Analysis
  

• Centrifuges

• Mircofuge

• Eppendorf  5415-C Operation  [TBW]

• Large Centrifuge

• Cell Culture Concentration
• IEC Centra-8 Manual

• pH Meters

• Hanna Checker hand-held pH meter

• Calibration and Operation [TBW, see manual]
• Hanna Checker Manual [scan or download]

• Hanna pH Meter and Ion Selective Electrodes

• pH Measuring [TBW]
• ISE Protocol - Calcium
• ISE Protocol - NH3
• NH3 ISE Manual

• Waste Disposal

• Waste Processing and Communication [ needs to address the topics below]
• Biohazards and Universal Precautions  [TBW]
• Sterilizing Liquid Media [TBW]
• pH Guidelines and Neutralization Processes for Liquid Waste  [TBW, see pH drain disposal - email exchange with EHS Director ]

• Labware Washing

• Manual washing
• Labconco Labware Washer
  

• Incubators

• Incubation Temperatures

• Bioreactor

• Bioreactor Setup and Operation

• Pipettes

• Micro-Pipettes Operation (.1uL-1000uL) [TBW]

• Videos

• Standard Pipettes (.1mL-25mL: 2mL, 10mL, 25mL) [TBW]

• Vortexers [TBW]
• Ultrasonic Bath [TBW]

Protocols for Common Media

• Bio Lab Common Media Spreadsheet
• Generic Liquid Media
• Whiffin Growth
• MICP
• PDYA Media [T

Protocols for Lab Activities

• Genetic Engineering

• Cloning with Restriction Enzymes

• Digestion+Ligation+Transformation Protocol

• CRISPR

• CRISPR Protocol (BioRad Blue/White Screening) [PDF on class Website, move to Lab drive and link]
  

• Microbial Drawing and Printing

• Agar Media Protocols

• Standard LB Agar [TBW from generic media protocol and media spreadsheet ]
• Black LB Agar [TBW, standard + activated charcoal]
• Cast 3D LB Agar [TBW standard with extra agar]

• Drawing  [TBW]
• Laser-cut stencils

• Protocol for Digitally Fabricating Stencils  [TBW]
• Grasshopper files [files on class website/picasso drive, move to google drive, link]
• Laser settings for Kapton [data in a rhino file, get kapton specs from McMaster Order]
• Protocols for Using Stencils

• Manual cell spreading [TBW]
• Ultrasonic Vapor Deposition of Cells [TBW, process needs to be refined]

• Microbial Cellulose

• Media

• HS Media for G. Hansenii [TBW from generic media protocol and media spreadsheet]
• Kombucha Media [TBW, printed handout in drawer in lab]

• Protocol for growing G. Hansenii in large tubs
• Post-Growth Processing

• Microbial Cellulose Rinsing Protocol
• Photopolymer Infiltration and Curing Protocol [TBW, from CCV report]
• Cyclomethicone Treatment Protocol [TBW]
• Standard Cellulose Drying Protocol
  

• Mycelium

• Mycelium Composite Casting Protocol
• Mycelium Leather [TBW, did Danielle ever write up her work?]
• Media

• PDYA Media [TBW from Penn State protocol]
• Liquid media  [TBW]

• Biocement

• Media

• Whiffin Growth
• MICP

• Bioreactor Setup and Operation
• Cell Culture Concentration
• Biocement Casting [TBW]
• Pumping System Programming and Operation [TBW]

• BW from Penn State protocol]