Standard Operating Procedure

Copper-64

Radiations emitted: Gamma & X-ray: 511 keV (36% abundance), 7-8 keV (34% abundance) Betas: 578 keV (37% abundance), 653 keV positron (18% abundance)

Shielding: Total absorption of beta radiation: 1.8 mm perspex Half value layer (HVL) for X and gamma rays 6 mm lead Tenth value layer (TVL) for X and gamma rays 17 mm lead

t½: 12.7 h

Radiolabeling process:

  1. Dilute Cu-64 solution into 0.05 M hydrochloric acid (HCl) to desired concentration or volume
  2. Suspend ligand into 0.25 M ammonium acetate buffer (NH4OAc) at the desired concentration in a vial
  3. Add appropriate concentration of activity to ligand
  4. Mix for 30 min at room temperature. Test labeling efficiency by HPLC or radio-TLC (20 mM EDTA/0.15 M NH4OAc). Free copper will travel with solvent.
  5. If free copper is still present stir of 60 min at 80 °C. Test labeling efficiency by HPLC or radio-TLC (20 mM EDTA/0.15 M NH4OAc).
  6. Ligand may be purified by Sep-Pak or HPLC.

Safety precautions

  1. Store 64Cu behind 2 in [~5 cm] thick lead (Pb) bricks - Use tools to indirectly handle unshielded sources and potentially contaminated vessels; avoid direct hand contact - Ensure that an appropriate, operational survey meter is present in the work area and turned on whenever 64Cu is handled, so that any external exposure issues will be immediately apparent and hence quickly addressed - Shield waste containers as needed to maintain accessible dose rate ALARA and < 2 mR/hr
  2. Maintain your occupational exposure to radiation As Low As Reasonably Achievable [ALARA].
  3. Ensure all persons handling radioactive material are trained, registered, & listed on an approved protocol.
  4. Review the protocol(s) authorizing the procedure to be performed and follow any additional precautions in the protocol.  Contact the responsible Principal Investigator to view the protocol information.
  5. Plan experiments to minimize external exposure by reducing exposure time, using shielding and increasing your distance from the radiation source.  Reduce internal and external radiation dose by monitoring the worker and the work area after each use of radioactive material, then promptly cleaning up any contamination discovered.  Use the smallest amount of radioisotope possible to minimize radiation dose and radioactive waste.  
  6. Keep an accurate inventory of radioactive material, including records of all receipts, transfers & disposal.  Perform and record regular lab surveys.
  7. Provide for safe disposal of radioactive waste by following institutional Waste Handling & Disposal Procedures.  Avoid generating mixed waste (combinations of radioactive, biological, and chemical waste). Note that lab staff may not pour measurable quantities of radioactive material down the drain.
  8. If there is a question regarding any aspect of the radiation safety program or radioactive material use, contact Radiation Safety.
  9. Disposable gloves, lab coats, and safety glasses are the minimum PPE [Personal Protective Equipment] required when handling radioactive material.  Remove & discard potentially contaminated PPE prior to leaving the area where radioactive material is used. Always wear radiation dosimetry monitoring badges [body & ring] whenever handling 64Cu
  10. Clearly outline radioactive material use areas with tape bearing the legend "radioactive".  Cover lab bench tops where radioactive material will be handled with plastic-backed absorbent paper; change this covering periodically and whenever it's contaminated.  Alternatively cover benches with thick plastic sheeting (i.e., painter’s drop cloth), periodically wipe it clean and replace it if torn.
  11. Label each unattended radioactive material container with the radioactive symbol, isotope, activity, and, except for waste.Place containers too small for such labels in larger labeled containers.  
  12. Handle radioactive solutions in trays large enough to contain the material in the event of a spill.
  13. Never eat, drink, smoke, handle contact lenses, apply cosmetics, or take/apply medicine in the lab; keep food, drinks, cosmetics, etc. out of the lab entirely.  Do not pipette by mouth.  
  14. Never store [human] food and beverage in refrigerators/freezers used for storing radioisotopes.
  15. Prevent skin contact with skin-absorbable solvents containing radioactive material.
  16. Fume hoods and biological safety cabinets for use with non-airborne radioactive material must be approved (through the protocol) and must be labeled "Caution Radioactive Material".
  17. Use sealed containers and appropriate secondary containment to carry radioactive material between rooms.