Hair regenerative medicine has emerged as a promising treatment for hair loss. We previously demonstrated that hair follicle germ (HFG)-like aggregates generated hair follicles when transplanted into mice. However, a challenge of this approach was to control the density and direction of hair shafts. Our recent study showed that hair follicle organoids sprouted hair shafts in vitro. This study proposes an approach to further grow the hairs to the length that can be handled with tweezers and transplanted into the skin. This approach may be useful for highly oriented hair regeneration.