Pictures:

Charts:

(There are actually two different charts, including velocity in the chart above!)

Graphs:

Questions:

How many subunits does the toothpickase have?

3

Where is your active site?

Aluminum Plate

What happens to the reaction rate as the supply of toothpicks runs out?

It goes down

What would happen to the reaction rate if the toothpicks were spread out so that the breaker has to reach for them?

The rate would go down

What would happen to the reaction rate if more toothpics (substrate) were added?

There would be more chances to break toothpicks and more toothpicks broken.

What would happen to the reaction rate if there were more “breakers” (more enzymes)?

It would go up, then down.

What would happen to the reaction rate if the “breaker” wears bulky gloves when picking toothpicks?

It would go down.

For each time-interval, calculate the rate of reaction of the toothpickase using the formula for the slope of the graph.

The rate of reaction is 3/20, 2/20, 3/20, -6/20, but 3/20 happened more.

Based on the table above, what is the Vmax of toothpickase?

The Vmax is .7

When was the reaction rate the slowst? The fastest?

5/70 and 5/80 ; 11/100

Explain the relationship between substrate concentration and reaction rate.

As the substrate concentration goes up, reaction rate increases, but the reaction rate became unstable after that.

Calculate the rate of enzyme action in toothpicks per second for each temperature. Show all of your work.

Krystal: 5,6,5,7,9; 5+6+5+7+9=32/5=6.4

Sarah: 6+6+4+6+8; 6+6+4+6+8=30/5=6

Compare the 2 rates. Explain what happened and why it happened.

The rate Krsytal broke toothpicks was 6.4 toothpicks per second. The rate Sarah brpke toothpicks was 6 toothpicks per second. The reason Krystal could probably break more toothpicks than Sarah was because the ice water probably had a greater effect on Sarah’s bony hands than Krystal’s strong hands.

Based on your results in Parts A, B, and C, discuss the rate of enzyme activity under various conditions.

For Part A, the number of toothpicks broken increased, then decreased. For Part B, the rate of enzyme activity is unsteady. For Part C, the rate of enzyme activity increases.