FEI Tomography        

2012aas, 2014vdb

For detailed description and help:

1. tomography acquisition guide.pdf -- Titan Help folder on the data computer

2.or Start the FEI tomo software and press F1 for help page

An Extremely simplified workflow for a complicated process

1. Starting the Tomography Software

        a) go to Experiment OCX (quick link on lower right)

        b) click TEM tomography component

        c) click TEM Tomography (or Batch Tomography)

        d) then click Start to start the software.

2. Adjust Filter: typical settings can be loaded from a saved file.

a) Use suppress peak at zero

b) add Hanning window

c) Long wavelength cutoff 20-200 nm (magnification dependent).

3. start data collection

a) find suitable locations for imaging (i.e. interesting structures and gold label concentrations)

b) the region of interest should be within 400um of the grid center in the y direction and not close to grid bars to obtain the maximum tilt range of -70 to +70 degrees

c)because the tilt axis of the Titan is horizontal areas can be imaged successfully further from the center along the x axis

4. Click Acquire Tilt Series taskbar

a) Choose camera settings- These settings for all four camera modes work well for most applications:

1. bin by 1

2. 1 second exposure

3. full CCD

4. other camera settings may also work, but should be tested in advance

B) click Proceed

1. fill in tilt series parameters

2. read the output information to follow progress

3. follow instructions

e) Check Correlation Peaks

1. watch display to be sure correlation peaks are found correctly by the software.

2. if so, the tracking should work well & a tilt series should be collected successfully.

4. For Batch Tomography,

a) click Prepare Batch to add regions of interests to a target list first

b) Make sure the cross correlation peak is found in the automated step of backlash correction.

1. This ensures that software can find the targeted location later after moving away from it

2. check /adjust the eucentric height of each region of interest at lower magnification in Search State, before adding it to the target list (then correct z height gets saved with the location)