Protocol for dipping/clipping capillaries

NOTES/TROUBLESHOOTING

1.  Set up LabVIEW programs (all 3) BEFORE mounting a capillary on the stage and taking out your poly-L-lysine (PLL) and gold nanoparticle aliquots from the fridge

2.  ALWAYS check the gold distribution on your tube for after coating it in PLL and gold

3.  Use a dust-blower to clean the diamond blade every now and then

4.  If there is an error message/pop-up window, try the following:

SETUP

1.  Turn on the power strip under the table

2.  Plug in power sources:

3.  Turn on the PC and monitor

4.  Log on as malegros (no password needed)

5.  Start menu > LabVIEW


LABVIEW: IMAG32at.VI

1.  Getting Started window > Open > C:\...Examples\LabVIEW\IMAG32at.VI

2.  Click Run

3.  Turn on Thorlabs LED driver

4.  Settings

5.  If the image is grainy, press either arrow button on the Thorlabs filter behind the microscope/camera lens

6.  Tools


LABVIEW: Hamiltoncontrol.vi

1.  File > Recent files > C:\...command\Hamiltoncontrol.vi

2.  Click Run

3.  Turn on pressure gauge to ~60 PSI

4.  **ONLY USE PINK, PURPLE AND ORANGE MODULES**

Function

Direction of movement

Micron values to use

PINK

Specimen (stage), camera, cutter positions

In/out

**Don’t use specimen up/down

PURPLE

Camera position

Micro X

(-) left;  (+) right

50-100

Micro Y

(-) down;  (+) up

DO NOT MOVE UNLESS YOU CAN’T SEE THE BLADE WHEN CAMERA AND CUTTER IN

Micro Z:  bring tube into focus

(-) and (+) are arbitrary

1-2

ORANGE

Capillary stage position

Specimen Y

(-) down;  (+) up

**Don’t use Specimen Z

25-3000

(use 500-1500 to find the tube)

5.  Test Camera in/out and Cutter in/out buttons to make sure everything is working

6.  Bring both Camera and Cutter (diamond blade) ‘in’


LABVIEW: myansupdown.vi

1.  File > Recent files > C:\...\malegros\Desktop\myansupdown.vi

2.  Default settings for poly-L-lysine coating:

PREP GOLD AND POLY-L-LYSINE SOLUTIONS

1.  Get gold and PLL aliquots from the fridge, then vortex the gold for 1 min.

2.  Fill eppie #1 to the top with PLL, then put it into the bottom left slot on the solution stage

3.  Fill eppie #2 with gold, then set it aside

POSITION THE CAPILLARY

1.  In IMAG32at.VI:

2. Mount the capillary onto the stage

3.  In Hamiltoncontrol.vi:

4.  Focus the tube by using the back knob for the camera/microscope

5.  Position the tube (using Specimen Y) to approximately the diameter at which you will cut it


DIPPING THE CAPILLARY INTO SOLUTION

1.  In Hamiltoncontrol.vi:

2.  In myansupdown.vi:

3.  Change the PLL eppie for the gold solution eppie on the solution stage

4.  In myansupdown.vi:

5.  In Hamiltoncontrol.vi:

6.  If you don’t see the tip in the IMAG32at.VI viewer, it’s broken. DISCARD THE TUBE.


CUTTING THE CAPILLARY TIP

1.  In Hamiltoncontrol.vi:

2.  In IMAG32at.VI:

3.  Turn the front knob counterclockwise to bring the cutter into focus (the edge will appear sharp)

4.  In IMAG32at.VI:

5. Turn the front knob clockwise to bring the cutter out of focus

6.  In Hamiltoncontrol.vi:

7.  In IMAG32at.VI:

8.  Turn the front knob counterclockwise again to bring the cutter into focus

9.  Flip the switch to cut the tip

10.  Turn the front knob clockwise to bring the cutter out (past the point of it being in focus)

11.  In Hamiltoncontrol.vi:


CHECK THE CAPILLARY TIP DIAMETER AND GOLD DISTRIBUTION

1.  Position the tube so that the very tip is approximately in the middle of the IMAG32at.VI viewer

2.  In IMAG32at.VI:

3.  CHECK THE GOLD DISTRIBUTION:

SHUTDOWN

1.  Exit all LabVIEW programs

2.  Turn pressure down to zero

3.  Shut down computer

4.  Unplug both Thorlabs and Stontronics power sources

5.  Turn off power strip