IB PCR Virtual Lab

The development of Polymerase Chain Reaction (PCR) in 1983 by Kary Mullis resulted in the a nobel prize for the inventor and a means to create thousands to millions of copies of a single region of DNA.  This process is now used in DNA cloning, fingerprinting, analysis of genes, DNA phylogeny, and other areas of science.  

Examine  Utah Genetic’s Virtual PCR Lab as an introduction to the technique and process of PCR

Introduction to PCR:

  1. Open the PCR Virtual Lab: http://learn.genetics.utah.edu/content/labs/pcr/
  2. What is the purpose of PCR?

  1. Where can DNA for PCR be obtained?

  1. What are the role of primers in PCR?  

  1. Why are there two types of primers?

  1. Why are nucleotides added to the PCR tube?

  1. What is the role of a thermocycler?

  1. What occurs at the following temperatures?
  1. 95 degrees Celsius:

  1. 50 degrees Celsius:

  1. 72 degrees Celsius:

  1. How does the process produce so many copies of a desired fragment of DNA?