Biochemistry
Lesson 1.3
Enzymes
“When you do the common things in life in an uncommon way, you will command the attention of the world.”
― George Washington Carver
Figure 1.3.9. Products that were developed by George Washington Carver and made available commercially. by Tuskegee University via US National Park Service: George Washington Carver National Monument / Public Domain
What helps chemical reactions take place in your cells?
Please read the above quote and look at the images on this page (Figures 1.3.9.). On the lines below, please write your ideas as to what you think this lesson is going to be about.
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1. Start by going through this interactive enzyme activity
2. What type of organic macromolecule are enzymes? __________________
3. Would our bodies function properly without enzymes? __________________
4. Are enzymes specific? ______________
5. Can enzymes be used over and over again? ______________
6. What can denature an enzyme? ______________ or ________________
7. Please draw a simple sketch that shows how enzymes are specific to certain substrates. Please label the enzyme and the substrate in your sketch.
8. Please draw a simple sketch that shows what happens when an enzyme is denatured. Please label the enzyme and the substrate in your sketch.
9. Follow the instructions on this website to complete the enzyme-controlled reactions virtual lab.
10. What is the purpose of this investigation?
11. Click on the TV/VCR to watch an animation about enzymes. Click on the blue “Information” button. Explain what is meant by the phrase: ““enzymes are organic catalysts.”
12. First, add the same amount of substrate to every test tube. How much substrate did you add to each of the five test tubes? ________
13. Keep the pH constant in each of the tubes. What is the pH in each tube? ________
14. The temperature should also be constant for all five test tubes. What is the temperature? __________
15. Click the computer monitor to see the data. What is your dependent variable? __________________ (What are you are measuring?)
16. What did you observe when you added the same amount of substrate to each test tube? How many molecules of product were formed per minute?___________________
17. Click the “reset” button. Add the amount of substrate laid out in front of each test tube. Click the computer monitor to see the data. What happens to the reaction rate when you increase the amount of substrate?
18. Click the “reset” button. Add the same amount of substrate to every test tube again. How much substrate did you add to each of the five test tubes? ________
19. Now increase the pH. What happens when you increase the pH?
20. What happens when you decrease the pH?
21. Adjust the pH of each test tube as shown below:
test tube 1 = pH 3
test tube 2 = pH 5
test tube 3 = pH 7
test tube 4 = pH 9
test tube 5 = pH 11
Add 1.0 g of substrate to each test tube. Click the computer monitor to see the data. What is the optimum pH for this reaction when 1.0 g of substrate is added to each test tube? ____________ How do you know? _______________________________________________________________________________
Please compare the vocabulary bubble maps you created at the beginning of the lesson with your completed vocabulary table definitions and extra practice. In the space below, please reflect on what you have learned so far in this lesson and how you have learned it.
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