Name:__________________________________________________________ Per:______Date:_______
Diffusion in Agar Cubes Pre-Lab
1.Which of the following shapes is a cube? Circle it.
2. Using the formula below, find the surface area of the following shapes. Show your work. All measurements are in centimeters.
Surface Area = Area (L x W) x # of sides
3. What is the formula for volume?
4. Using the formula above, find the volume of the following shapes. Again, all measurements are in centimeters.
5. What is the surface area to volume ratio of cube A (SA:V)? ___:___
SA V
6. Express the above ratio in its simplest form. _______________
7. What is the surface area to volume ratio of cube B (SA:V)? ) ___:___
SA V
8. Express the above ratio in its simplest form. _______________
9. Which of the two ratios is the smallest? __________________
Diffusion In Agar Cubes
Is Bigger Better? OR Is Smaller Smarter?
Adapted from Flinn Scientific Publication No. 10110
Introduction:
Diffusion is one of the very important processes by which substances such as nutrients, water, oxygen, and cellular wasted are transported between living cells and their environment. This activity will help you explore the relationship between diffusion and cell size by experimenting with model “cells.”
In this experiment, you will use agar cubes to which the indicator phenolphthalein has been added. Phenolphthalein is an acid/base indicator that turns pink in the presence of a base such as NaOH. Thus the surface of the agar cubes will turn pink immediately when put into a NaOH solution. The NaOH will continue to diffuse through the cube and gradually turn the inside of the cube pink. The guiding question for this lab is thus:
Investigative Question: What determines the efficiency of diffusion throughout the model “cells”?
Use this question to help formulate a hypothesis:
Materials:
Agar
A ruler
0.1M NaOH per group 200mL
Knife
Large beaker
Spoon/Tongs
Procedure:
Calculating % diffusion in each cube:
Calculate the surface area of each cube and the surface area to volume ratio:
Conclusion Questions: In your logbook answer the following questions. Be sure to tape data table on top of your hypothesis and conclusion questions.
DATA TABLE: Diffusion In Agar Cubes Name:_____________________________
Cube Size | Total cube volume (cm3) | Total volume that was not pink (cm3) | Sketch of each Cube | Volume of the diffused cube (total volume – volume that was not pink) | PercentDiffusion | Surface area of cube (cm2) | Surface area to volume ratio |
1cm | |||||||
2cm | |||||||
3cm |
DATA TABLE: Diffusion In Agar Cubes Name:______________________________
Cube Size | Total cube volume (cm3) | Total volume that was not pink (cm3) | Sketch of each Cube | Volume of the diffused cube (total volume – volume that was not pink) | PercentDiffusion | Surface area of cube (cm2) | Surface area to volume ratio |
1cm | |||||||
2cm | |||||||
3cm |
Preparation of agar