Unit 5: DNA - Blue

6 Classes

Dates

Day

IB Topic Number

Activities

Objectives

Homework

11/26 & 11/27

26

2.5

Mitosis Day

  • Cancer exploration
  • Cell Cycle Introduction
  • Begin Cell Division Mitosis Lab
  • Mutagens, oncogenes and metastasis are involved in the development of primary and secondary tumors
  • Application: The correlation between smoking and incidence of cancers.
  • Mitosis is division of the nucleus into two genetically identical daughter nuclei
  • Chromosomes condense by supercoiling during mitosis
  • Cytokinesis occurs after mitosis and is different in plant and animal cells
  • Interphase is a very active phase of the cell cycle with many processes occurring in the nucleus and cytoplasm
  • Cyclins are involved in the control of the cell cycle

Cell Division Lecture & Notes 1.6

Reading: 228-235 &  240-243

Enzyme Lab

11/28 & 11/29

27

2.5

Cell Division & DNA Structure Day

  • Quiz: 1.6 Cell Division
  • Cell Division Mitosis Lab

  • Skill: Determination of a mitotic index from a micrograph.
  • Skill: Identification of phases of mitosis in cells viewed with a microscope or in a micrograph.

DNA Structure Lecture & Notes, 2.6

Reading: 86-89

11/30 & 12/3

28

DNA Structure Day

  • Quiz: 2.6 DNA Structure
  • DNA Structure Drawings
  • DNA Structure Model Lab
  • HIstory of DNA Structure & Discovery

  • DNA is a double helix made of two antiparallel strands of nucleotides linked by hydrogen bonding between complementary base pairs
  • The nucleic acids DNA and RNA are polymers of nucleotides
  • DNA differs from RNA in the number of strands present, the base composition and the type of pentose
  • Skill: Drawing simple diagrams of the structure of single nucleotides of DNA and RNA, using circles, pentagons and rectangles to represent phosphates, pentoses and bases.
  • Application: Tandem repeats are used in DNA profiling.
  • Application: Rosalind Franklin’s and Maurice Wilkins’ investigation of DNA structure by X-ray diffraction.

DNA Replication Lecture

Reading: 313-322

12/4 & 12/5

29

DNA Replication Day

  • Quiz: 7.1 Quiz
  • Replication Explanation
  • Animations
  • Introns & Extrons
  • Gel Electrophoresis Virtual Lab & Introduction

  • The replication of DNA is semi-conservative and depends on complementary base pairing
  • Helicase unwinds the double helix and separates the two strands by breaking hydrogen bonds
  • DNA polymerase links nucleotide together to form a new strand, using the preexisting strand as a template
  • DNA polymerases can only add nucleotides to the 3’ end of a primer.
  • DNA replication is continuous on the leading strand and discontinuous on the lagging strand.
  • DNA replication is carried out by a complex system of enzymes.
  • Nucleosomes help to supercoil the DNA
  • DNA structure suggested a mechanism for DNA replication.

Gel Electrophoresis Virtual Lab & Introduction

12/6 & 12/7

30

DNA Replication Day

  • Replication Lab
  • Vocabulary Concept Map
  • Replication Practice
  • Enzyme Lab Corrections

  • Application: Use of nucleotides containing deoxyribonucleic acid to stop DNA replication in preparation of samples for base sequencing.
  • Application: Tandem repeats are used in DNA profiling.
  • Some regions of DNA do not code for proteins but have other important.
  • Skill: Analysis of results of the Hershey and Chase experiment providing evidence that DNA is the genetic material.
  • Skill: Utilization of molecular visualization software to analyse the association between protein and DNA within a nucleosome.
  • Skill: Analysis of Meselson and Stahl’s results to obtain support for theory of semi-conservative replication of DNA
  • Application: Production of human insulin in bacteria as an example of the universality of the genetic code allowing gene transfer between species.
  • Skill: Use a table of the genetic code to deduce which codon(s) corresponds to which amino acid.

12/10 & 12/11

31

Gel Electrophoresis of Dye samples

Hypothesis writing practice

Writing a procedure activity

DNA Profiling

Gel Electrophoresis Virtual Lab Due 12/10

12/11 DNA Retakes Deadline

12/12 & 12/13

32

DNA Assessment

Exploration Practice 1 

2.7/7.2 Transcription Notes

Reading: 331-334