Negative Selection TolC 7/11

	B	C	D	E	F	G	H	I	J
1
2		1	2	3	4	5	6	7	8
3	A	Ancestor Positive	Ancestor Positive Vancomycin	A9 Postive	A9 Postive Vancomycin	A11 Postive	A11 Postive Vancomycin	D8 Postive	D8 Postive Vancomycin
4	B	Ancestor Negative	Ancestor Negative Vancomycin	A9 Negative	A9 Postive Vancomycin	A11 Negative	A11 Postive Vancomycin	D8 Negative	D8 Postive Vancomycin
5	C	All are 1:100 Cells that have been growing overnight (1.5uL Culture) 150uL Lbmin in all wells (1.5uL Vancomycin) 1.5uL colicin E1
6	D
7	E
8	F
9	G
10	H
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