Plasmids_KC_Lab

	A	B	C	D	E	F	G	H	I
1	plasmid Name	Vector Backbone	Insert	Necessary Information	Storage place Plasmid	Glycerol Stock Strain	Plasmid map	Concerned Person	Plasmid Source
2	pym-14	pym-14	nil	Euroscarf	-20	Dh5 alpha-80		Raj
3	pym-N-22	Pym-N-22	nil	Euroscarf	-20	Dh5 alpha-80		Raj
4	pym-N-16	pym-n-16	nil	euroscarf	-20	Dh5 alpha-80		raj
5	pym-N-21	pym-N-21	nil	euroscarf	-20	Dh5 alpha-80		Raj
6	PRS-PGH	PRS-PGH	nil	euroscarf	-20	Dh5 alpha-80		Raj
7	PRS-420	PRS-420	nil	euroscarf	-20	Dh5 alpha-80		raj
8	pym-n23	pym-n23	nil	euroscarf	-20	dh5 alpha-80		Raj
9	pym-25	pym-25	nil	euroscarf	-20	Dh5 alpha-80		Raj
10	pym-36	pym-36	nil	euroscarf	-20	Dh5 alpha-80		Raj
11	pym-43	pym-43	nil	euroscarf	-20	Dh5 alpha-80		raj
12	pym-35	pym-35	nil	euroscarf	-20	dh5 alpha-80		Raj
13	PNG	PNG	nil	Addgene	-20	dh5 alpha-80		Raj
14	PNI	PNI	nil	Addgene	-20	Dh5 alpha-80		raj
15	PHD	PHD	nil	Addgene	-20	dh5 alpha-80		Raj
16	PNN	PNN	nil	Addgene	-20	dh5 alpha-80		Raj
17	pPM47	pPM47	UPRE1-RFP	addgene	-20	Dh5 alpha-80		Raj
18	pPM48	pPM48	EROGFP-UPRE1RFP	addgene	-20	Dh5 alpha-80		Raj
19	pPM-56	pPM-56	EROGFP-UPRE1RFP	addgene	-20	Dh5 alpha-80		raj
20	pBF3038	pBF3038	CRE Recombinase	addgene	-20	Dh5 alpha-80		Raj
21	Pym-N23 CYC1min	Pymn23	CYC1 min	CYC1 min region from yeast	-20	Dh5 alpha-80		Raj
22	Pym-N23 CYC1min Dsred	Pymn23	CYC1 min Dsred	Dsred Cloned downstream of Cyc1 min	-20	Dh5 alpha-80		raj
23	Pym-N23-Kar2ss Cyc1	Pymn23	Kar2ss Cyc1	Kar2 signal sequence and cyc1 terminator	-20	dh5 alpha-80		Raj
24	Pym-N23 -Dm-MBP-Kar2sscyc1HDEL	Pym-N23	DM-MBP downstream of GALpr followed by cyc1termintor and ER Rention Seq HDEL		-20	Dh5 alpha-80		Raj
25	Pym-N23 -Sm-MBP-Kar2sscyc1HDEL	Pym-N23	SM-MBP downstream of GALpr followed by cyc1termintor and ER Rention Seq HDEL		-20	Dh5 alpha-80		Raj
26	Pym-N23 -Ss-MBP-Kar2sscyc1HDEL	Pym-N23	Ss-MBP downstream of GALpr followed by cyc1termintor and ER Rention Seq HDEL		-20	Dh5 alpha-80		raj
27	Pym-N23 -D95c-MBP-Kar2sscyc1HDEL	Pym-N23	D95c-MBP downstream of GALpr followed by cyc1termintor and ER Rention Seq HDEL		-20	dh5 alpha-80		Raj
28	Lss-mkate	Puc	M-kate		-20	dh5 alpha-80		Raj
29	Lss-mkate2	puc	M-kate-2		-20	Dh5 alpha-80		raj
30	Hsp104pr-N23	Pym-N23	Hsp104pr		-20	dh5 alpha-80		Raj
31	Hsp104prLssmate-N23	Pym-n23	lssmate	lssmkate Downstream of HSP104pr	-20	dh5 alpha-80		Raj
32	Hsp104prBFPe-N23	Pym-N23	BFP	BFP downstream of Hsp104pr	-20	dh5 alpha-80		Raj
33	Hsp104prBFP2 micron-N23	Pym-N23	2 micron	2 micron	-20	dh5 alpha-80		Raj
34	GPD-M25	Pym 25	GFP pr	GPDpr	-20	Dh5 alpha-80		raj
35	Pym-25 delta GFP	pym-25	GFP deleted		-20	dh5 alpha-80		Raj
36	IREGFP.M25	pym-25	IRE1 Luminal	Ire1 Luminal Upstream of GFP	-20	dh5 alpha-80		Raj
37	IREGFPIREM25	pym-25	IRE1 Luminal	Ire1 Luminal Upstream of GFP and Downstream of GFP	-20	dh5 alpha-80		Raj
38	PRS-PGH-NATRFP	PRS-PGH	NAT RFP		-20	dh5 alpha-80		Raj
39	PRS316	PRS316			-20	dh5 alpha-80		Raj
40
41	pYM-N23-ER-PBMD	pYM-N23-kar2ss	Kar2 signal sequence	DMMBP in opposite orientation	-20	dh5 alpha-80		Kannan
42	pYM-N23-MM-PBMD	pYM-N23-cyb2delta19	MM signal sequence	for mitochondrial matrix localization	-20	dh5 alpha-80		Kannan
43	pYM-N23-Nucl-PBMD	pYM-N23-NLS	NLS signal sequence	for Nuclear localization	-20	dh5 alpha-80		Kannan
44	pYM-N23-Cyto-PBMD	pYM-N23	No signal seq	for cytoplasmic localization	-20	dh5 alpha-80		Kannan
45	pYM-N23-IMS-PBMD	pYM-N23-cyb2	IMS signal sequence	for mitochondrial intermembrance space localization	-20	dh5 alpha-80		Kannan
46	pYM-N23-ER-WtDHFR	pYM-N23-kar2ss	Kar2 signal sequence	Wild type Dihydrofolate reductase ampified from Su-DHFR plasmid	-20	dh5 alpha-80		Kannan
47	pYM-N23-MM-WtDHFR	pYM-N23-cyb2delta19	MM signal sequence	for mitochondrial matrix localization	-20	dh5 alpha-80		Kannan
48	pYM-N23-Nucl-WtDHFR	pYM-N23-NLS	NLS signal sequence	for Nuclear localization	-20	dh5 alpha-80		Kannan
49	pYM-N23-Cyto-WtDHFR	pYM-N23	No signal seq	for cytoplasmic localization	-20	dh5 alpha-80		Kannan
50	pYM-N23-IMS-WtDHFR	pYM-N23-cyb2	IMS signal sequence	for mitochondrial intermembrance space localization	-20	dh5 alpha-80		Kannan
51	pYM-N23-ER-mutCDHFR	pYM-N23-kar2ss	Kar2 signal sequence	mutant C Dihydrofolate reductase(DHFR) ampified from Su-DHFR plasmid	-20	dh5 alpha-80	Gowda et al., PNAS (pg.5975–5980) 2013	Kannan
52	pYM-N23-MM-mutCDHFR	pYM-N23-cyb2delta19	MM signal sequence	for mitochondrial matrix localization	-20	dh5 alpha-80		Kannan
53	pYM-N23-Nucl-mutCDHFR	pYM-N23-NLS	NLS signal sequence	for Nuclear localization	-20	dh5 alpha-80		Kannan
54	pYM-N23-Cyto-mutCDHFR	pYM-N23	No signal seq	for cytoplasmic localization	-20	dh5 alpha-80		Kannan
55	pYM-N23-IMS-mutCDHFR	pYM-N23-cyb2	IMS signal sequence	for mitochondrial intermembrance space localization	-20	dh5 alpha-80		Kannan
56	pYM-N23-MM-PBMD-GFP	pYM-N23-cyb2delta19	MM signal sequence	for mitochondrial localization study	-20	dh5 alpha-80		Kannan
57	pYM-N23-IMS-PBMD-GFP	pYM-N23-cyb2	IMS signal sequence	for mitochondrial intermembrane localization study	-20	dh5 alpha-80		Kannan
58	pVT-100U-GFP	-	MM signal sequence	GFP targeting to mitochondrial matrix	-20	dh5 alpha-80		Kannan
59	pYES2-Gal1	-	-	Gal1 promoter-Cyc terminator	-20	dh5 alpha-80		Kannan
60	pPS189	pPS189	nil	URA; TEF; 2u for over expression studies	-20	dh5 alpha-80		Kannan	IISc
61	pJV340	pJV340	nil	LEU; TEF; 2u for over expression studies	-20	dh5 alpha-80		Kannan	IISc
62	pRS316-mutC	pRS316	-	mutant form of DHFR cloned in pRS316 vector	-20	dh5 alpha-80		Kannan	Gowda et al., PNAS (pg.5975–5980) 2013
63	pRS316-mutD	pRS316	-
64	pYES-DEST52	pYES-DEST52	nil	For Gateway clonning, destination vector	-20		pYES-DEST52	Rohan	invitrogen
65
66
67	pRS426 _with GPD promoter	pRS426	nil	has ura marker, 2u, GPD promoter, cyc1 terminator	-20	dh5alpha	http://www.atcc.org/products/all/87361.aspx	asmita	JNU
68	pRS426 _with TEF promoter	pRS426	nil	has ura marker, 2u, TEF promoter, cyc1 terminator	-20	dh5alpha		asmita	JNU
69	pRS423	pRS423	nil	has his marker, 2u	-20	dh5alpha	http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pRS423/	asmita	JNU
70
71	HSF1		HSF1 of yeast with promoter of HSF1	ura marker, HSF1 of yeast with promoter of HSF1	-20	dh5alpha		asmita	Winge lab
72	R206S		HSF1 of yeast containg a mutation at 206 position, R to S; making it activewith promoter of HSF1	ura marker, active HSF1 of yeast with promoter of HSF1	-20	dh5alpha		asmita	Winge lab
73	wt nat	pRS316	wt nat	ura marker, centromeric	-20	dh5alpha		asmita	made by Anu / latika
74	TS 4	pRS316	ts 4 nat	ura marker, centromeric	-20	dh5alpha		asmita	made by Anu / latika
75	TS 5	pRS316	ts 5 nat	ura marker, centromeric	-20	dh5alpha		asmita	made by Anu / latika
76	TS 15	pRS316	ts 15 nat	ura marker, centromeric	-20	dh5alpha		asmita	made by Anu / latika
77	TS 22	pRS316	ts 22 nat	ura marker, centromeric	-20	dh5alpha		asmita	made by Anu / latika
78	PXH1853 SKN7 (WT) (myc2x)	PRS315	yeast skn7 native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
79	PXH1854 skn7-T437A (myc2x)	PRS315	yeast skn7 native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
80	PXH1855 skn7-T455A (myc2x	PRS315	yeast skn7native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
81	PXH1856 skn7-T449A (myc2x)	PRS315	yeast skn7native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
82	PXH1859 SKN7 (WT) (GFP	PRS315	yeast skn7native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
83	PXH1860 skn7-T437A (GFP	PRS315	yeast skn7native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
84	PXH1861 skn7-T455A (GFP	PRS315	yeast skn7native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
85	PXH1862 skn7-T449A (GFP)	PRS315	yeast skn7native Promoter and Terminator	leu2 marker	-20	dh5alpha		raj	university of iowa,USA
86	PWO0510(CPY*)	pRS316	yeast cpy*(mutated)native Promoter and Terminator	ura3 marker	-20	dh5alpha		raj	Wolf lab germany
87	PwO1049(CPY)	pRS316	yeast cpy(WT)native Promoter and Terminator	ura3 marker	-20	dh5alpha		raj	Wolf lab germany
88	PRS316_cup1_mPRDX4_WT	pRS316	PRDX4 with CUP1 promoter and cyc1 terminator	ura3 marker	-20	dh5alpha		raj	David ron lab, cambridge
89	PRS316_cup1_mPRDX4_c127S	pRS316	PRDX4 with CUP1 promoter and cyc1 terminator	ura3 marker	-20	dh5alpha		raj	David ron lab, cambridge
90	PRS316_cup1_mPRDX4_c248s	pRS316	PRDX4 with CUP1 promoter and cyc1 terminator	ura3 marker	-20	dh5alpha		raj	David ron lab, cambridge
91	ER MBP	pYM-N23	Nat-Gal promoter-Kar2ss-MBP-hdel cyc1 term		-20	dh5alpha		Pratima	PCR tool box
92	Cyto MBP	pYM-N23	Nat-Gal promoter-MBP-hdel cyc1 term		-20	dh5alpha		Pratima	PCR tool box
93	MM MBP	pYM-N23	Nat-Gal promoter-cybdel19ss-MBP-hdel cyc1 term	truncated cytochrome b50;cybdelta19 signal sequence, for targeting to Mitochondrial matrix (MM) in yeast	-20	dh5alpha		Pratima	PCR tool box
94	IMS MBP	pYM-N23	Nat-Gal promoter-Cybss-MBP-hdel cyc1 term	cytochrome b50 (cyb full length) signal sequence for targeting to Intermembrane space (IMS) in yeast	-20	dh5alpha		Pratima	PCR tool box
95	ER DMMBP	pYM-N23	Nat-Gal promoter-Kar2ss-DMMBP-hdel cyc1 term		-20	dh5alpha		Pratima	PCR tool box
96	Cyto DMMBP	pYM-N23	Nat-Gal promoter-DMMBP-hdel cyc1 term		-20	dh5alpha		Pratima	PCR tool box
97	Nuc DMMBP	pYM-N23	Nat-Gal promoter-NLS-MBP-hdel cyc1 term	Nuclear localising signal (NLS) for targeting to nucleuss in yeast	-20	dh5alpha		Pratima	PCR tool box
98	MM DMMBP	pYM-N23	Nat-Gal promoter-Cybdel19ss-DMMBP-hdel cyc1 term		-20	dh5alpha		Pratima	PCR tool box
99	IMS DMMBP	pYM-N23	Nat-Gal promoter-Cybss-DMMBP-hdel cyc1 term		-20	dh5alpha		Pratima	PCR tool box
100	p426-GPD	p426	nil	yeast episomal shuttle vector with 2u circle, GPD promoter, 6606 bp plasmid	-20	dh5alpha		Pratima	from Asmita