Lab Practicum #1 Objectives
Basic Terminology
1. Define the anatomical position and know the anatomical terms used to describe various regions of the body.Histology
Be
able to identify, under the microscope, each of the specific tissues
listed below, and the additional structures indicated in bold-face
type. Additionally, for each tissue, be able to give examples of
their locations in the body The
page numbers refer to the Saladin/Wise 2nd edition, lab manual. For other lab manuals, just look in the index for the applicable pages. And don't
forget to use your textbook as well! The Virtual Histology Lab Practical on Dr. C’s website is also a useful resource. (Note: you are not responsible for slides 1 & 2)
1. Epithelial
Tissue
Simple columnar epithelium: slide 3 (page 37).
Simple
cuboidal
epithelium: slides 3,4 (page 36).
Simple squamous
epithelium; slide 4 (look for it lining blood vessels on the
slide) (page 35).
Stratified squamous
epithelium: slides 5, 22, 23 (page 38).
Pseudostratified
ciliated columnar epithelium: slides 10, 6; notice the goblet
cells and cilia (page 37).
2. Connective
tissue (CT)
Areolar
CT: slide 7; note the elastic fibers, collagen fibers,
and fibroblasts (page 45).
Adipose tissue: slides 9, 22, 23; note the cell nuclei, cytoplasm, and intracellular fat (page 45).
Dense regular CT: slide 8 (page 43).
Dense
irregular CT: slide 23 (page 43).
Hyaline cartilage: slides 6, 10; note the chondrocytes in lacunae. There are also lots of collagen fibers in the matrix, although they can't be seen (page 47).
Fibrocartilage:
slide 11; note the chondrocytes
in lacunae and the collagen fibers in the tissue
matrix (page 47).
Elastic cartilage: slide 12;
note chondrocytes
in lacunae and the elastic fibers in the tissue
matrix (page 48).
Bone (osseous) tissue: slide
13; note the Haversian
systems (osteons),
including the Haversian
(central) canal, lacunae, canaliculi,
matrix, and lamellae within each system (pages 48, 73). Also, identify
the these
structures on the bone tissue model, as well as blood vessels,
nerves, and osteocytes.
This model is also on-line at Dr.
C.'s website (tinyurl.com/56wpr7).
Blood: slide 14: note the erythrocytes, leukocytes (neutrophils, eosinophils, basophils, lymphocytes, monocytes), and platelets (pages 49, 283-285).
3. Muscle
Tissue
Skeletal
muscle tissue: slides 15,18;
note striations and nuclei of the skeletal muscle
cells and the occasional groupings of adipose cells (page
40).
Smooth muscle tissue: slides 16,18 (page 41).
Cardiac muscle tissue: slides 17, 18; note the
branching cardiac muscle cells with intercalated disks (the
disks may not be visible on some slides) (page 41).
4. Nerve
Tissue
Neurons
and glial
cells: slide 19; note a neuron and its nucleus, axon
hillock, axon, and dendrites; also note the glial
cells. (pages 43, 202)
Myelinated
nerve fibers: slide 20; note nodes of Ranvier,
and Schwann
cells (neurolemmocytes)
covering axons (see page 218; although the photograph there doesn't much look like this slide, it will help you identify the nodes of Ranvier. The online histology quiz at Dr. C.'s website has
a photo that does look like the slide (at tinyurl.com/67u8qp).
White matter
of spinal cord: slide 21; note myelinated
axons in cross section (page 236)
Grey matter
of spinal cord: slide 21; note neuron cell bodies and the
dense network of dendrites (page 236)